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KMID : 1094720210260040568
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Biotechnology and Bioprocess Engineering
2021 Volume.26 No. 4 p.568 ~ p.574
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Suppressive Activities of Fisetin on Particulate Matter-induced Oxidative Stress
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Sim Hyun-Chae
Noh Ye-Eun
Choo Sam-Yeol
Kim Na-Yeon
Lee Tae-Ho
Bae Jong-Sup
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Abstract
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Exposure to high levels of atmospheric particulate matter (PM) with an aerodynamic diameter of less than 2.5 μm (PM2.5) causes respiratory injury mainly due to oxidative stress. Although the fisetin has biological activities such as the antiviral, neuroprotective, and anti-inflammatory activities, the effect of fisetin on PM-mediated oxidative damage has not been studied. In this study, we tested the protective effect of fisetin against PM2.5-induced toxicity in human pulmonary artery endothelial cells (HPAECs) and its molecular mechanism. Exposure to PM2.5 decreased cell viability in HPAECs in a time- and dose-dependent manner, possibly due to increased release of extracellular lactate dehydrogenase and generation of intracellular reactive oxygen species (ROS). Cell viability assay demonstrated that treatment of HPAECs with fisetin increased cell viability and reduced PM2.5-induced oxidative stress in a dose-dependent manner. Serum- and glucocorticoid-inducible kinase 1 (SGK1), a crucial cell survival factor, was downregulated by PM2.5 which was recovered by fisetin. Furthermore, fisetin treatment inhibited intracellular ROS in HPAECs generated by PM2.5. Moreover, decreased antioxidant enzymes activities of superoxide dismutase and catalase level in PM2.5-treated cells were reversed by fisetin treatment. Our results suggest that fisetin effectively protects human HPAECs from PM2.5-induced oxidative damage via antioxidant effects.
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KEYWORD
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fisetin, particulate matter, oxidation, ROS
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